Computer-Assisted Transgenesis of Caenorhabditis elegans for Deep Phenotyping
نویسندگان
چکیده
منابع مشابه
Computer-Assisted Transgenesis of Caenorhabditis elegans for Deep Phenotyping
A major goal in the study of human diseases is to assign functions to genes or genetic variants. The model organism Caenorhabditis elegans provides a powerful tool because homologs of many human genes are identifiable, and large collections of genetic vectors and mutant strains are available. However, the delivery of such vector libraries into mutant strains remains a long-standing experimental...
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The ability to manipulate the genome of organisms at will is perhaps the single most useful ability for the study of biological systems. Techniques for the generation of transgenics in the nematode Caenorhabditis elegans became available in the late 1980s. Since then, improvements to the original approach have been made to address specific limitations with transgene expression, expand on the re...
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چکیده انگیزه دلیل اصلی رفتارهای ما است. به نظر می رسد انگیزه جزء جدایی ناپذیر فرایند یادگیری باشد. ارزش ذاتی موفقیت تمایل به پیشرفت را در یادگیرنده ایجاد میکند. به عبارت ساده این تمایل انگیزه پیشرفت نامیده میشود. انگیزه پیشرفت را میتوان در احساس یادگیرنده هنگام چالش با درس های مدرسه، لذت انجام فعالیت درسی، یا حس کشف پاسخ مشاهده کرد.حتی ممکن است انگیزه پیشرفت را در تلاش یادگیرنده برای جلب تایید...
Computer Assisted Assembly of Connectomes from Electron Micrographs: Application to Caenorhabditis elegans
A rate-limiting step in determining a connectome, the set of all synaptic connections in a nervous system, is extraction of the relevant information from serial electron micrographs. Here we introduce a software application, Elegance, that speeds acquisition of the minimal dataset necessary, allowing the discovery of new connectomes. We have used Elegance to obtain new connectivity data in the ...
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We employed the Tag-seq technique to generate global transcription profiles for different strains and life stages of the nematode C. elegans. Tag-seq generates cDNA tags as does Serial Analysis of Gene Expression (SAGE), but the method yields a much larger number of tags, generating much larger data sets than SAGE. We examined differences in the performance of SAGE and Tag-seq by comparing gene...
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ژورنال
عنوان ژورنال: Genetics
سال: 2015
ISSN: 1943-2631
DOI: 10.1534/genetics.115.179648